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Cholesterol depletion with MβCD enhances BoNT/A induced neuroparalysis in adult NMPs and shortens the time to inhibit stimulus-evoked EPCs. A, Samples traces of EPCs for adult diaphragm NMPs treated with BoNT/A (10 pM; 90 min) either following αCD (top panel) or MβCD (bottom panel) at 0, 40, or 90 min of BoNT/A exposure. B, Bar graphs summarize the mean EPC amplitude ± SD for the above conditions for n = 6 mice, 11 NMPs, and 22 endplates.
