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. 2017 Jun 22;159(1):179–188. doi: 10.1093/toxsci/kfx127

Figure 9.

Figure 9

Cholesterol supplementation via Ch-MβCD with water soluble cholesterol delays the time to inhibit 50% twitch tension in adult diaphragm NMPs. A. Time courses of stimulus evoked twitch tension in adult diaphragm NMPs treated with either αCD alone (10 mM; 30 min; control), or BoNT/A with and without either MβCD (10 mM; for 30 min preincubation) or MβCD saturated with cholesterol (10 mM; present throughout the experiment). B, Bar graphs represent the mean time to inhibit 50% of stimulus evoked twitch tension ± SEM followed by BoNT/A (10 pM) under the conditions mentioned earlier (n = 5–8 NMPs for each condition).