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. 2017 Oct 13;161(2):310–320. doi: 10.1093/toxsci/kfx215

Figure 4.

Figure 4.

TCDD, 11-Cl-BBQ, ITE, and FICZ treatment leads to Tr1 cell differentiation on day 2 of the alloresponse. Spleen and peripheral lymph node cells from B6 mice were labeled with CFSE and transferred into F1 host mice (day 0). Host mice were treated with TCDD (15 μg/kg on day 0), 11-Cl-BBQ (7.5 mg/kg on days 0 and 1, BBQ), ITE (40 mg/kg every 6 h on days 0 and 1), FICZ (10 mg/kg on days 0 and 1), or FICZ (50 μg/kg on days 0 and 1, FICZlow). On day 2, splenocytes were stained and analyzed for (A) CD4 expression on donor cells, (B) donor CD4+ T cell proliferation, and (C) CD25 expression on donor CD4+ cells. The donor CD4+CD25+ population was then analyzed for the percentage of (D) CTLA4+CD62Llow, (E) CCR9+, and (F) CCR4+ cells. Each point represents an individual mouse. Treatment groups with different letters are significantly different. p ≤ .05.