Figure 5.

Effects of siRNA-based kinase knockdown on hiPSC CM action potentials and Ca²⁺and contractility transients. Each trace shows representative recordings of membrane potential, cytosolic Ca²⁺ and contraction made 72 h posttransfection with either non-silencing control siRNA (siControl, black traces) or a pool of siRNA targeted against the indicated kinase gene (red traces). The level of RNA knockdown achieved in each experiment were 83%, 49%, 83%, 76%, 77%, 74%, 32%, 57% (percent of control, top to bottom respectively). *P ≤ .05, **P ≤ .005, ***P ≤ .0005—when compared with siControl using ANOVA and Bonferroni post hoc.