Table 1.
Kinases Identified by a Univariate Analysis With SMKI-Induced Effects on hiPSC CM
| Kinase | Group | Enrichment | MCC | P-value | |
|---|---|---|---|---|---|
| Expressed in hiPSC CM and heart | PAK1 | STE | 3.06 | 0.50 | 1.0E-04 |
| RPS6KB1 | AGC | 2.84 | 0.56 | 0.0E+00 | |
| MST4 | STE | 2.82 | 0.49 | 1.6E-04 | |
| RPS6KA3 | AGC | 2.63 | 0.49 | 2.4E-04 | |
| FAK | TK | 2.63 | 0.49 | 8.0E-05 | |
| PAK3 | STE | 2.55 | 0.42 | 1.2E-03 | |
| MAP3K4 | STE | 2.55 | 0.42 | 1.0E-03 | |
| STK33 | CAMK | 2.54 | 0.56 | 0.0E+00 | |
| MAP4K2 | STE | 2.50 | 0.43 | 6.4E-04 | |
| FER | TK | 2.47 | 0.49 | 1.6E-04 | |
| MAP3K12 | TKL | 2.47 | 0.49 | 1.0E-04 | |
| STK35 | OTHER | 2.47 | 0.49 | 1.8E-04 | |
| MAP3K2 | STE | 2.44 | 0.44 | 4.6E-04 | |
| MAP4K3 | STE | 2.39 | 0.46 | 3.8E-04 | |
| PIP4K2B | LIPID | 2.36 | 0.41 | 1.6E-03 | |
| PRKAA2 | CAMK | 2.36 | 0.41 | 1.3E-03 | |
| PIM3 | CAMK | 2.36 | 0.41 | 1.3E-03 | |
| SGK3 | AGC | 2.36 | 0.41 | 1.5E-03 | |
| STK24 | STE | 2.36 | 0.41 | 1.3E-03 | |
| PRKCQ | AGC | 2.36 | 0.59 | 0.0E+00 | |
| PRKD3 | CAMK | 2.34 | 0.49 | 1.0E-04 | |
| TBK1 | OTHER | 2.34 | 0.49 | 1.2E-04 | |
| CAMKK2 | OTHER | 2.34 | 0.49 | 1.0E-04 | |
| MAP4K1 | STE | 2.32 | 0.45 | 2.4E-04 | |
| CDK7 | CMGC | 2.27 | 0.47 | 2.0E-04 | |
| ROCK1 | AGC | 2.22 | 0.41 | 1.3E-03 | |
| PRKD1 | CAMK | 2.22 | 0.41 | 1.5E-03 | |
| INSR | TK | 2.22 | 0.41 | 1.5E-03 | |
| MAP2K3 | STE | 2.22 | 0.41 | 1.4E-03 | |
| NUAK1 | CAMK | 2.22 | 0.41 | 1.5E-03 | |
| LATS2 | AGC | 2.22 | 0.41 | 1.2E-03 | |
| CAMK1D | CAMK | 2.22 | 0.41 | 1.4E-03 | |
| SRPK3 | CMGC | 2.22 | 0.41 | 1.4E-03 | |
| ULK2 | OTHER | 2.19 | 0.52 | 6.0E-05 | |
| PRKD2 | CAMK | 2.17 | 0.46 | 3.2E-04 | |
| CHEK2 | CAMK | 2.17 | 0.46 | 4.2E-04 | |
| PRKCE | AGC | 2.17 | 0.46 | 3.8E-04 | |
| NUAK2 | CAMK | 2.14 | 0.50 | 1.0E-04 | |
| PRKX | AGC | 2.12 | 0.42 | 1.8E-03 | |
| PRKACA | AGC | 2.12 | 0.42 | 1.9E-03 | |
| ZAP70 | TK | 2.12 | 0.42 | 1.7E-03 | |
| PRKCD | AGC | 2.12 | 0.52 | 6.0E-05 | |
| PRKG1 | AGC | 2.12 | 0.52 | 2.0E-05 | |
| STK4 | STE | 2.10 | 0.42 | 1.3E-03 | |
| IKBKE | OTHER | 2.10 | 0.42 | 9.8E-04 | |
| PKN2 | AGC | 2.02 | 0.50 | 4.0E-05 | |
| Expressed in heart, not in hiPSC CM | SYK | TK | 2.36 | 0.41 | 1.5E-03 |
| AKT2 | AGC | 2.12 | 0.42 | 1.8E-03 | |
| FES | TK | 2.10 | 0.42 | 1.2E-03 | |
| Not expressed in hiPSC CM or heart | MINK1 | STE | 2.71 | 0.51 | 4.0E-05 |
| JAK3 | TK | 2.65 | 0.52 | 4.0E-05 | |
| GRK1 | AGC | 2.47 | 0.49 | 8.0E-05 | |
| YSK4 | STE | 2.45 | 0.46 | 4.0E-05 | |
| GRK7 | AGC | 2.23 | 0.49 | 1.0E-04 | |
| CAMKK1 | OTHER | 2.10 | 0.42 | 1.3E-03 | |
| HIPK1 | CMGC | 2.53 | 0.46 | 3.6E-04 | |
| MAP3K13 | TKL | 2.47 | 0.49 | 1.4E-04 | |
| LATS1 | AGC | 2.17 | 0.46 | 4.0E-04 |
Fifty-eight kinases in total were identified as statistically significant; 46 are expressed in adult heart and hiPSC CM at time of culture for experimentation; 3 kinases are expressed in heart but not in hiPSC CM; 9 kinases are not expressed in either hiPSC CM or adult heart.