Table 2.
Individual Kinases Discovered as Critical for CM Function
| Amplitude |
Beat Rate |
|||
|---|---|---|---|---|
| Kinase | % siControl | FDR-Adjusted P-value | % siControl | FDR-Adjusted P-value |
| CAMKK2 | — | — | 86.3 | 2.3E–05 |
| CDK7 | 76.8 | 2.1E–04 | — | — |
| FAK | 79.2 | 4.0E–05 | 88.5 | 1.4E–03 |
| FER | 79.1 | 2.2E–04 | 88.4 | 1.2E–03 |
| IKBKE | — | — | 66.8 | 1.7E–06 |
| MAP3K12 | 78.5 | 4.1E–04 | 76.0 | 7.6E–09 |
| MAP3K2 | 72.1 | 4.5E–07 | — | — |
| MAP3K4 | 62.1 | 2.3E–07 | 84.9 | 6.9E–05 |
| MAP4K1 | 71.6 | 4.0E–05 | 91.4 | 2.9E–03 |
| MAP4K2 | 57.1 | 2.0E–10 | — | — |
| MST4 | 70.5 | 2.2E–06 | — | — |
| NUAK1 | 85.7 | 3.5E–03 | — | — |
| PAK1 | 59.1 | 1.7E–10 | — | — |
| PAK3 | 67.0 | 4.9E–09 | 119.5 | 1.1E–06 |
| PIP4K2B | 58.8 | 1.2E–10 | — | — |
| PRKAA2 | 51.3 | 8.7E–13 | — | — |
| PRKACA | 83.7 | 6.6E–04 | 109.2 | 2.7E–03 |
| PRKCD | 71.0 | 1.1E–06 | 64.3 | 1.1E–11 |
| PRKCE | — | — | 90.1 | 2.1E–03 |
| PRKCQ | 73.1 | 6.6E–07 | — | — |
| PRKD2 | 76.9 | 2.5E–05 | — | — |
| PRKG1 | 73.7 | 7.7E–08 | — | — |
| ROCK1 | 85.1 | 5.2E–03 | — | — |
| RPS6KA3 | 64.4 | 3.1E–08 | — | — |
| STK4 | 72.6 | 3.3E–07 | 89.0 | 9.4E–04 |
| STK24 | — | — | 86.0 | 3.9E–06 |
| STK35 | 73.0 | 1.7E–06 | — | — |
| TBK1 | 66.0 | 2.7E–09 | — | — |
| ULK2 | 76.4 | 1.6E–05 | — | — |
| ZAP70 | 78.8 | 9.6E–04 | 90.5 | 2.1E–03 |
The effect of siRNA-based knockdown on beat rate or amplitude expressed as % siControl is listed for each kinase. “—” indicates no significant change compared with siControl. Only those kinases for which there was a significant change in beat rate or amplitude upon knockdown (FDR-adjusted P < .01) are listed in this table. A complete description of knockdown effects on beat rate, beat amplitude, cell index, and cellular ATP level is listed for all 45 genes knocked down in Supplementary Table 3.