Lung-specific overexpression of IKK2ca induces systemic and adipose but not hypothalamic inflammation. A, B, Plasma were harvested from normal diet-fed WT and LungIKK2ca mice (10–18-weeks old, n = 5/group), and their TNFα and IL-6 were measured by ELISA. C-H, WT, and LungIKK2ca mice were fed with doxycycline diet for 3 months. C, D, Plasma TNFα and IL-6 were measured by ELISA. E, F, TNFα and IL-6 mRNA in the epididymal adipose tissue were measured by qPCR. G, H, TNFα and IL-6 mRNA in the hypothalamus were measured by qPCR (n = 6 or 8). *p < .05 versus WT, student t test.