Lung-specific overexpression of IKK2ca induces endothelial dysfunction. A, B, Thoracic aorta were harvested from normal diet-fed WT and LungIKK2ca mice (10–18-weeks old, n = 5/group). A, Their contractile responses to PE. B, They were precontracted by PE (1 µM) and then relaxed by Ach. C–F, WT and LungIKK2ca mice were fed with doxycycline diet for 3 months. C, Aortic contractile response to PE. D, Aortic rings were precontracted by PE (1 µM) and then relaxed by Ach. E, Aortic contractile response to PE in the presence of NOS inhibitor L-NAME. F, Aortic rings were precontracted by PE and then relaxed by NO donor, SNP (n = 6 or 8). *p < .05 versus WT, 2-way ANOVA.