Figure 1.

Experimental protocol for treatment and behavior assessment of zebrafish larvae. Zebrafish were plated at 6–8 h postfertilization, and then dosed at 6-days postfertilization (dpf), immediately before assessment. Locomotor activity was recorded in the dark for 60 min, and analysis software was used to derive distance moved (cm)/unit time (min). Control (unshaded wells) and treated (shaded wells) fish were dosed in alternating columns of each 96-well plate.