Table 1. Measurement of SLURP1 in human serum by EIA.
| CUSABIO-EIA | Cloud-Clone/USCN-EIA | |
|---|---|---|
| General Information | ||
| Immunogen‘s production | CHO cell line (eukaryotic) | E. coli bacteria (prokaryotic) |
| Immunogen/Standard | full-length recombinant protein | full-length recombinant protein |
| Posttranslational modifications (e.g., disulfide bonding) | abundant | limited |
| Coating antibody | mouse monoclonal IgG | mouse monoclonal IgG |
| Detecting antibody | biotin-labeled mouse monoclonal IgG | biotin-labeled rabbit polyclonal IgG |
| Detecting reagent/substrate | avidin-HRPO/TMB | avidin-HRPO/TMB |
| Standard detection range | 5–300 ng/ml | 0.156-10 ng/ml |
| Dilution of sera samples | 1:2 | 1:10 |
| Measurements | ||
| No. of patients: | 106 | 67 |
| Donor | 23 ± 4 ng/ml n = 17 |
11 ± 3 ng/ml n = 15 |
| CP | 27 ± 3 ng/ml n = 26 |
13 ± 3 ng/ml n = 26 |
| PDAC | 28 ± 3 ng/ml n = 63 |
10 ± 3 ng/ml n = 26 |
| Frequency of SLURP1- negative serum: | ||
| Donor | 0% | 27% |
| CP | 0% | 27% |
| PDAC | 0% | 23% |
| PDAC- pN0 | 0% | 56% |
| PDAC- pN1 | 0% | 6% |
| Standards’ inter-EIA cross-reactivity: | ||
| CUSABIO-Standard | 150 ng/ml | 10 ng/ml |
| USCN-Standard | 0 ng/ml | 10 ng/ml |