Table 1. Proteins identified by nano-HPLC-Chip ion trap MS/MS.
| Protein name | Gene name | Spot no. a) (Supplementary Figure 1) | NCBI acc. # |
No. peptides identified | Mascot score | Mr. (Da) exp./theor. | pI exp./theor. | Seq. Coverage (%) |
Fold change b) | p (corr) |
p value (< 0.01) |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Apoptosis: | |||||||||||
| 10 kDa heat shock protein, mitochondrial | HSPE1 | 9004 | gi|4504523 | 18 | 443 | 10000/10925 | 8.8/8.89 | 76 | 2.07 | 0.879 | 0.0016 |
| adenylate kinase 2, mitochondrial | AK2 | 8202 | gi|4502013 | 5 | 206 | 29000/26745 | 7.8/7.67 | 21 | 1.59 | 0.831 | 0.0043 |
| Co-Chaperone P23 | PTGES3 | 102 | gi|9257073 | 4 | 112 | 20000/15216 | 4.5/5.09 | 36 | −10 | −0.774 | 0.0056 |
| cyclophilin A | PPIA | 8103 | gi|1633054 | 9 | 270 | 17500/18154 | 7.8/7.82 | 35 | 1.54 | 0.885 | 0.0001 |
| manganese superoxide dismutase | SOD2 | 7101 | gi|62738405 | 7 | 214 | 22000/21864 | 7.3/6.86 | 27 | 1.64 | 0.909 | 0.0007 |
| MICOS complex subunit MIC19 | CHCHD3 | 9102 | gi|8923390 | 3 | 135 | 27000/26491 | 8.4/8.48 | 8 | 1.82 | 0.884 | 0.0007 |
| mitochondrial single-stranded DNA binding protein | SSBP1 | 8002 | gi|2624694 | 5 | 163 | 15500/15186 | 7.8/8.23 | 36 | 1.77 | −0.809 | 0.0002 |
| profilin-1 | PFN1 | 9002 | gi|4826898 | 4 | 207 | 15000/15258 | 8.4/8.44 | 37 | 1.41 | 0.804 | 0.0089 |
| profilin-1 | PFN1 | 9001 | gi|4826898 | 7 | 193 | 15000/15258 | 8.4/8.44 | 49 | 1.96 | 0.978 | 0.0000 |
| protein DJ-1 | PARK7 | 4108 | gi|31543380 | 6 | 166 | 25000/20092 | 5.9/6.33 | 33 | −1.56 | −0.799 | 0.0038 |
| voltage-dependent anion-selective channel protein 1 | VDAC1 | 8307 | gi|4507879 | 18 | 455 | 35000/30896 | 8.0/8.62 | 43 | 1.79 | 0.915 | 0.0004 |
| Cell migration, invasion, and metastasis: | |||||||||||
| alpha-enolase | ENO1 | 5610 | gi|4503571 | 20 | 641 | 50000/47566 | 6.8/7.01 | 37 | −1.54 | −0.904 | 0.0004 |
| alpha-enolase | ENO1 | 5608 | gi|4503571 | 13 | 492 | 50000/47566 | 6.7/7.01 | 33 | −1.54 | −0.904 | 0.0002 |
| B23 nucleophosmin | NPM1 | 1406 | gi|825671 | 6 | 294 | 36000/31132 | 4.7/4.71 | 25 | −1.72 | / | 0.0021 |
| heat shock protein beta-1 | HSPB1 | 5201 | gi|4504517 | 12 | 208 | 28000/22840 | 6.2/5.98 | 33 | 1.31 | 0.869 | 0.0004 |
| nuclear corepressor KAP-1 | NCOR1 | 902 | gi|1699027 | 2 | 79 | 90000/90682 | 4.6/5.52 | 3 | −6.25 | −0.810 | 0.0082 |
| phosphatidylethanolamine-binding protein 1 | PEBP1 | 7107 | gi|4505621 | 3 | 197 | 21000/21186 | 7.6/7.01 | 22 | 1.39 | 0.895 | 0.0012 |
| transgelin-2 | TAGLN2 | 9101 | gi|4507357 | 10 | 288 | 21000/22605 | 8.4/8.41 | 32 | 1.30 | 0.878 | 0.0024 |
| Oxidative stress: | |||||||||||
| albumin | ALB | 4708 | gi|119626083 | 4 | 110 | 60000/58614 | 5.8/6.66 | 6 | 1.87 | 0.832 | 0.0018 |
| ATP synthase subunit d, mitochondrial | ATP5H | 2103 | gi|5453559 | 4 | 143 | 21000/18551 | 5.2/5.21 | 16 | 1.55 | 0.900 | 0.0002 |
| malate dehydrogenase | MDH2 | 9307 | gi|2906146 | 9 | 454 | 36000/36077 | 9.0/8.92 | 28 | 2.95 | 0.786 | 0.0085 |
| peroxiredoxin-1 | PRDX1 | 8107 | gi|4505591 | 13 | 379 | 23000/22096 | 8.0/8.27 | 42 | 1.42 | 0.865 | 0.0030 |
| mRNA processing and translation: | |||||||||||
| 40S ribosomal protein S21 | RPS21 | 7004 | gi|4506699 | 2 | 93 | 10000/9248 | 7.6/8.68 | 33 | 1.61 | 0.927 | 0.0003 |
| elongation factor Tu | EFTUD | 6501 | gi|704416 | 9 | 294 | 49000/49935 | 6.9/7.7 | 22 | 1.58 | 0.814 | 0.0078 |
| heterogeneous nuclear ribonucleoproteins A2/B1 | HNRPA2B1 | 9312 | gi|4504447 | 4 | 233 | 35000/35984 | 8.2/8.67 | 14 | 2.33 | / | 0.0000 |
| heterogeneous nuclear ribonucleoproteins A2/B1 | HNRPA2B1 | 9313 | gi|4504447 | 4 | 140 | 35000/35984 | 8.6/8.67 | 14 | 2.54 | 0.869 | 0.0018 |
| heterogeneous nuclear ribonucleoproteins A2/B1 | HNRPA2B1 | 8306 | gi|4504447 | 4 | 250 | 33000/36055 | 7.9/8.67 | 14 | 2.59 | 0.868 | 0.0014 |
| Cytoskeleton organization: | |||||||||||
| alpha-tubulin | TUBA | 2707 | gi|37492 | 5 | 186 | 55000/50978 | 5.2/5.02 | 14 | −1.33 | −0.837 | 0.0057 |
| stathmin 1 | STMN1 | 4101 | gi|197692339 | 6 | 202 | 17500/17320 | 5.7/5.76 | 29 | −1.49 | −0.813 | 0.0040 |
a) Spot numbers refer to those in Supplementary Figure 1 b) Fold change: the expression ratios between the means of spots value (% vol) of control and AsA treated MeWo cells resulting from digital image analysis. – Indicates decreased protein intensity.