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. 2018 Feb 5;141(3):673–687. doi: 10.1093/brain/awx375

Figure 1.

Figure 1

HDAC6 co-immunoprecipitates with GlyRS. (A) N2a cells were transiently transfected with either pCMV-DDK-tagged human cDNA encoding GlyRS or pCMV-dsRed-tagged human cDNA encoding HDAC6. Co-immunoprecipitation was performed using agarose beads conjugated to an antibody directed against the DDK peptide. Transfected N2a cells were pre-treated with 1 µM tubastatin A (TubA) or vehicle (DMSO). Western blot analysis was performed to check for equal protein expression and co-immunoprecipitation of HDAC6. (B) N2a cells were transiently transfected with either pCMV-DDK-tagged human cDNA encoding wild-type or C175R mutant GlyRS or pCMV-dsRed-tagged human cDNA encoding HDAC6. Co-immunoprecipitation was performed using agarose beads conjugated to an antibody directed against the DDK peptide. Transfected N2a cells were pretreated with 1 µM tubastatin A or vehicle (DMSO). Western blot analysis was performed to check for equal protein expression and co-immunoprecipitation of HDAC6.