Table 1B.

Design of experiment to assess effects of exogenous DOX dose on elastic matrix neoassembly and proteolytic activity in cytokine (TNF-α and IL-1β; 10 ng/ml each)-injured EaRASMC cultures. Outcomes were measured in harvested cell layers at 21 days post-seeding.

Case	Cell Amount/ Type/# Cultures	Day 7 Serum Starve: 3 h Treatment: 30 min	Treatment Day 8 and Day 14 (Till Harvest on Day 21)
		1% FBS	10% FBS
Healthy Cell Controls	30K, RASMC, n=3	–	–
Treatment Controls	30K, EaRASMC, n=3	Cytokines	Cytokines
DOX (0.1 μg/mL)	30K, EaRASMC, n=3	DOX, Cytokines	DOX, Cytokines
DOX (1 μg/mL)	30K, EaRASMC, n=3	DOX, Cytokines	DOX, Cytokines
DOX (10 μg/mL)	30K, EaRASMC, n=3	DOX, Cytokines	DOX, Cytokines
DOX (20 μg/mL)	30K, EaRASMC, n=3	DOX, Cytokines	DOX, Cytokines
DOX (50 μg/mL)	30K, EaRASMC, n=3	DOX, Cytokines	DOX, Cytokines