. Author manuscript; available in PMC: 2019 Aug 1.

Published in final edited form as: Drug Deliv Transl Res. 2018 Aug;8(4):964–984. doi: 10.1007/s13346-017-0419-y

Table 2B.

Design of experiment to assess effects of DOX NPs on elastic matrix neoassembly and proteolytic activity in cytokine (TNF-α and IL-1β; 10 ng/ml each)-injured EaRASMC cultures. Outcomes were measured in harvested cell layers at 21 days post-seeding.

Case	Cell Amount/ Type/# Cultures	Day 7 Serum Starve: 3 h Treatment: 30 min	Treatment Day 8 and Day 14 (Till Harvest on Day 21)
Case	Cell Amount/ Type/# Cultures	1% FBS	10% FBS
Healthy Cell Controls	30K, RASMC, n=3	–	–
Treatment Controls	30K, EaRASMC, n=3	Cytokines	Cytokines
Blank NPs	30K, EaRASMC, n=3	Blank NPs, Cytokines	Blank NPs, Cytokines
DOX NPs	30K, EaRASMC, n=3	DOX NPs, Cytokines	DOX NPs, Cytokines