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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Insect Mol Biol. 2018 Jan 4;27(2):268–278. doi: 10.1111/imb.12370

Figure 2.

Figure 2

RNAi-mediated knockdown of AaKr-h1 reduced egg production in Ae. aegypti mosquitoes. (A) The expression of AaKr-h1 was successfully knocked down in the dsKr-h1 injected mosquitoes. Newly emerged adult female mosquitoes were injected with dsRNAs for AaKr-h1 or GFP (as control). A group of three mosquitoes was randomly picked from the un-injected, dsGFP- and dsKr-h1-injected mosquitoes at 96 h PE. The mRNA levels of AaKr-h1 were measured by real-time PCR. Results are the mean ± S.D. of three replicates. Statistical analysis was conducted by a paired t-test (**, p<0.01). (B) Knockdown of AaKr-h1 was confirmed by Western Blot. Proteins were extracted from the un-injected, dsGFP- and dsKr-h1-injected mosquitoes at 96 h PE. Western Blot analysis was conducted using anti-AaKr-h1 antibody. Anti-GAPDH antibody was used for the loading control. (C) Effect of the AaKr-h1 knockdown on the previtellogenic growth of primary follicles. Follicle lengths were measured at 96 h PE. Each bar represents mean ± S.D. of five independent measurements of follicles from ten mosquitoes in each group. Statistical analysis was conducted by a paired t-test. (D) Egg production after blood feeding in the AaKr-h1 RNAi mosquitoes. Dots represent egg counts for individual mosquitoes within 5 days after the blood meal. Lines represent mean numbers of eggs oviposited from three replicates; bars indicate the standard error of the mean (SEM). Data were analyzed using GraphPad software. Statistical analysis was conducted using a paired t-test (***, p<0.001).