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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Insect Mol Biol. 2018 Jan 4;27(2):268–278. doi: 10.1111/imb.12370

Figure 5.

Figure 5

RNAi-mediated knockdown of AaKr-h1 led to the downregulation of AAEL005810, AAEL013177, and AAEL005957. (A) The mRNA profiles of these three AaKr-h1 target genes in the fat body of previtellogenic female mosquitoes. Total RNA was extracted and the expression of AaKr-h1 target genes was measured using real-time PCR. (B) Expression of the AaKr-h1 target genes in the AaKr-h1-depleted mosquitoes. Newly emerged female mosquitoes were injected with dsRNAs for AaKr-h1 or GFP within 30 min after eclosion. At 96 h PE, fat bodies were dissected for total RNA extraction. The amount of individual transcripts in the dsKr-h1-injected and control mosquitoes was measured by quantitative RT-PCR. Data represent mean ± S.D. of three replicates. Statistical analysis was conducted using a paired t-test (*, p<0.05; **, p<0.01).