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. Author manuscript; available in PMC: 2019 Apr 1.
Published in final edited form as: Insect Mol Biol. 2018 Jan 4;27(2):268–278. doi: 10.1111/imb.12370

Figure 6.

Figure 6

AaKr-h1 acted as a repressor for the transcription of AAEL014226, AAEL004444, and AAEL005545 in the previtellogenic mosquitoes. (A) Expression of the three AaKr-h1 target genes in the fat body of previtellogenic female mosquitoes. The amounts of specific mRNA were measured using real-time PCR. (B) RNAi-mediated depletion of AaKr-h1 increased the expression of AAEL014226, AAEL004444 and AAEL005545. Newly emerged female mosquitoes were injected with dsRNAs for AaKr-h1 or GFP within 30 min after eclosion. The amount of individual transcripts, in the dsKr-h1-injected and control mosquitoes at 96 h PE in the fat body, was measured by quantitative RT-PCR. Data represent mean ± S.D. of three replicates. Statistical analysis was conducted using a paired t-test (*, p<0.05; **, p<0.01).