Figure 4.

Inhibition of SFKs impairs the stability of the SFKs-TRAF6-IRF-1-cIAP2 complex (A–C) hTLR7-HEK293 cells were pretreated or not with PP2 (20 µM) for 30 min and stimulated for 2 h with R848 (10 µM). Total cell lysates were immunoprecipitated using an anti-TRAF6 (A), anti-interferon regulatory factor 1 (IRF-1) (B), or anti-pSFKs (C) antibody, respectively. Co-immunoprecipitated cIAP2, TNFR-associated factor 6 (TRAF6), SFKs, and IRF-1 proteins were detected by immunoblot. Histograms on the right side of the blots show the quantification of the relative amount of the pulled-down proteins, calculated on three independent experiments. Each signal was normalized to the signal of the protein used for the pull-down and expressed as fold induction (mean ± SD) compared to untreated sample. ***P < 0.001, **P < 0.01, *P < 0.05.