Table 2.

Analysis of duodenal biopsies from control subjects, untreated CeD patients, and CeD patients subjected to gluten withdrawal.

Antigen	Primary antibody	Secondary antibody
Gal-1	In-house rabbit anti-Gal1 antibody (1:500) (64)	anti-rabbit biotinylated antibody (1:130) (Amersham Pharmacia, Buckinghamshire, UK)
Gal-4	Goat anti-Gal-4 antibody (1:75) (Santa Cruz Biotech, Dallas, TX, USA)	anti-goat biotinylated antibody (1:180) (Amersham Pharmacia, Buckinghamshire, UK)
Foxp3	Rabbit anti-Foxp3 antibody (1:50) (Abcam, Cambridge, UK)	anti-rabbit biotinylated antibody (1:130) (Amersham Pharmacia, Buckinghamshire, UK)

Antibodies used for immunohistochemical analysis of Gal-1, Gal-4, and Foxp3 expression in duodenal biopsies from control subjects and patients.

Four intestinal biopsies from the second duodenal section from each patient and control were collected.