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. 2018 Mar 1;9:362. doi: 10.3389/fimmu.2018.00362

Figure 2.

Figure 2

DR3 stimulation increases FoxP3+ Tregs without altering TH17 cell population during chronic ileitis. (A) Flow-cytometric analysis of mesenteric lymph node cells from IgG- and 4C12-treated SAMP or AKR mice after staining with specific anti-CD4 and anti-FoxP3 Abs or (C) anti-CD4 and anti-IL17 Abs. (B) Relative expression of FoxP3 mRNA and (D) of IL-17A mRNA was measured in total tissue RNA extracted from the terminal ilea of SAMP or AKR mice treated with IgG or 4C12 (10-week-old, n = 4). The relative expression of each target gene was normalized to the relative expression of β-actin in the sample. Data presented as median ± interquartile range and analyzed by two-way ANOVA, with Bonferroni’s post hoc test. Data are representative of three independent experiments.