Figure 7.

Facilitation of immune-related molecules expression by B4GALT5 over-expression in 3D4/21 cells. (A) The expression of Flag-B4GALT5 in 3D4/21 cells. 3D4/21 cells were transfected with Flag-B4GALT5 or empty vector as negative control. The Flag-B4GALT5 and empty vector were detected with a mouse anti-Flag mAb. β-actin was detected as an internal control using a mouse anti-β-actin mAb. (B–E) Analysis of transcription and expression of different cytokines produced by macrophages via qRT-PCR. (F–H) Protein expression levels of MHC-I, II by flow cytometry analysis and western blot. All data represented the means and standard deviation of three independent experiments. Differences in data were taken for be statistically significant if the P-value was less than 0.05 (^*p < 0.05; ^**p < 0.01; ^****p < 0.0001).