Figure 5.

ESCRT-III proteins association increase during phagocytosis. (A) Immunoprecipitation using α-rEhVps32 antibodies. Immunoprecipitates were submitted to western blotting assays using antibodies indicated at left. At right: molecular weight of immunodetected proteins. PS: pre-immune serum. Input: trophozoite lysates before immunoprecipitation. (B) Association of four couples of ESCRT-III proteins signaled in X-axis in trophozoites in basal conditions and during phagocytosis using the corresponding primary and secondary antibodies. Association was quantified by flow cytometry as described in section Materials and Methods. ^*p < 0.1, ^**p < 0.01, ^***p < 0.001.