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. 2018 Mar 1;8:53. doi: 10.3389/fcimb.2018.00053

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Copyright © 2018 Avalos-Padilla, Knorr, Javier-Reyna, García-Rivera, Lipowsky, Dimova and Orozco.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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EhVps20 active form binds more efficiently to GUVs than EhVps32. (A) GUVs prepared from POPC (62 mol%), POPS (10 mol%), chol (25 mol%), PI(3)P (3 mol%), and TR-DHPE (0.1 mol%) were incubated with 125 nM of each Alexa488 labeled recombinant protein indicated at left for 5 min. GUVs were analyzed through confocal microscope. Scale bar: 10 μm. (B) Co-localization of recombinant proteins with Texas-Red labeled membrane in 100 GUVs. Bars indicate average Pearson's coefficient. AU, arbitrary units.