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. 2018 Mar 1;8:53. doi: 10.3389/fcimb.2018.00053

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Copyright © 2018 Avalos-Padilla, Knorr, Javier-Reyna, García-Rivera, Lipowsky, Dimova and Orozco.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Knock down of EhVps20 gene affects the rate of erythrophagocytosis. Trophozoites of G3 clone were transfected with pSAP2/GunmaEhVps20 plasmid (EhVps20⁻) or with the empty vector (vector). (A) Western blot of transfected trophozoites lysates, using antibodies indicated at left. As a loading control, the same membrane was re-blotted with α-actin antibodies. (B) Densitometry analysis of bands showed in (A). (C) Representative image of confocal microscopy and phase contrast of transfected trophozoites using α-rEhVps20 antibody and TRITC-labeled secondary antibody. (D) Fluorescence intensity measured by pixels. (E) Novikoff stained trophozoites that ingested erythrocytes for 15 min. Scale bar: 10 μm. (F) Rate of erythrophagocytosis of transfected trophozoites. Data represent the mean and standard error of the erythrocytes number counted inside of 100 trophozoites. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001.