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Formation of three-dimensional hBM-MSC spheroids in Matrigel scaffold. (A) Phase contrast microscopy of hBM-MSC spheroids in Matrigel. Scale bar: 1,000 μm. (B–E) A representative hBM-MSC spheroid in phase contrast (B), with the nuclear stain Hoechst blue (C), stained with an anti-CD90 specific antibody red (D), and merge (E). Scale bar: 100 μm.
