Figure 3.

Association of gametocytes with hBM-MSC spheroids in the 3D co-culture system. (A) Phase contrast microscopy showing stage II gametocytes associated with hBM-MSC spheroids in Matrigel scaffold compared with (B) gametocytes randomly distributed in Matrigel scaffold without cells. Scale bar: 200 μm. (C) Three representative co-culture spheroids stained with anti-CD90 antibodies (red) to detect mesenchymal cells, anti-Pfg27 antibodies (green) to detect gametocytes and Hoechst (blue) to stain nuclei. Scale bar: 50 μm.