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. 2018 Feb 5;8(5):2594–2606. doi: 10.1002/ece3.3868

Table 2.

Adapter and primer sequences for AFLP and MSAP amplification

Primer	Sequence(from 5'to 3’)
Adapters
EcoRI_adapter top	CTCGTAGACTGCGTACC
EcoRI_adapter bottom	AATTGGTACGCAGTCTAC
MseI_adapter top	GAGCGATGAGTCCTGAG
MseI_adapter bottom	TACTCAGGACTCAT
HpaII/MspI_adapter top	GATCATGAGTCCTGCT
HpaII/MspI_adapter bottom	CGAGCAGGACTCATGA
Preselective primers
EcoRI+A	GACTGCGTACCAATTCA
MseI+C	GATGAGTCCTGAGTAAC
HpaII/MspI	ATCATGAGTCCTGCTCGG
Selective primers
EcoRI+AAC⁴	GACTGCGTACCAATTCAAC
EcoRI+ACA¹	GACTGCGTACCAATTCACA
EcoRI+ACT^2,6	GACTGCGTACCAATTCACT
EcoRI+ACC³	GACTGCGTACCAATTCACC
EcoRI+ACG⁵	GACTGCGTACCAATTCACG
MseI+CAT²	GATGAGTCCTGAGTAACAT
MseI+CTA¹	GATGAGTCCTGAGTAACTA
MseI+CTC³	GATGAGTCCTGAGTAACTC
HpaII/MspI+TA⁶	ATCATGAGTCCTGCTCGGTA
HpaII/MspI+TC^4,5	ATCATGAGTCCTGCTCGGTC

Superscript numbers indicate primer combinations used for the selective amplification, and every primer combination is tagged with same number.