Figure 4.

Cell GFP plasmid transfection efficiency and total fluorescence using electrospray in different sequences (a): 25 µl (1) 25 µl + 25 µl (2 × 25 µl) and 25 µl + 25 µl + 25 µl (3 × 25 µl). Flow cytometer fluorescence measurements were chosen for cell transfection efficiency and total fluorescence representation. Cells were sprayed with sucrose-based media with osmolarity of 370 mOsm. Working distance of 4 mm, flow rate of 20 µl/min with voltage of 3 kV was used for electrospray. Analysis was performed 24 hours later. Effect on transfection efficiency and total fluorescence of changing voltage (b) or working distance (c) with electrospray mediated pMaxGFP plasmid. Cells were sprayed with sucrose based media with osmolarity of 370 mOsm. Voltage range from 2.7 kV to 3.3 kV was used for electrospray (b), and working distance from 4 mm to 6 mm (c) was evaluated.