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. 2018 Mar 5;8:4014. doi: 10.1038/s41598-018-22383-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of in-utero protein restriction, accelerated postnatal growth and ageing upon expression of insulin signaling molecules in para-ovarian fat of 3 and 6 month female rats (a) IRβ protein expression (b) pIRβ^Y1361 protein expression, (c) Irs1 mRNA expression (d) IRS1 protein expression (e) Pkc-ζ mRNA expression, (f) PKC-ζ protein expression, (g) Total Akt protein expression and (h) pAkt^ser473 protein expression. Results are expressed as mean ± S.E.M. F2C = control; R = recuperated. *p < 0.05, **p < 0.01 and ***p < 0.001. N values: mRNA analysis = 7 per group. Protein analysis: 3 m C = 7, 3 m recuperated = 7, 6 m control = 6, 6 m recuperated = 5.