Table 1.
Rates of scarless editing at the HPRT locus using engineered microhomology
| Parent clone | Munich mutation | Enrichment | Samples analyzed | Normal allele | NHEJ (perfect) | Scarless excisiona | Fidelity (%)b | Silent ONLY | Munich & Silent |
|---|---|---|---|---|---|---|---|---|---|
| 033-B-43 | bilateral | HAT | 0 | n/a | n/a | n/a | n/a | n/a | n/a |
| no HAT | 179 | 0 | 171 (75) | 8 | 4.5 | 0 | 8 | ||
| 033-U-45 | unilateral | HAT | 49 | 0 | 0 | 49 | 100 | 49 | 0 |
| no HAT | 206 | 0 | 192 (84) | 14 | 6.8 | 5 | 9 |
arepair events without any additional base mutations, as predicted to occur via MMEJ
bcalculated as (Scarless excision/Samples analyzed)