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. 2018 Mar 5;8:4000. doi: 10.1038/s41598-018-22398-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cedratvirus getuliensis entry in Acanthamoeba castellanii cells. (A) Scanning microscopy showing a C. getuliensis particle attached to an Acanthamoeba cell. (B) The impact of different inhibitors of endocytic pathways in C. getuliensis entry. Treatment of amoebas with cytochalasin D reduced Cedratvirus getuliensis virion incorporation, indicating that particles can enter amoebas by phagocytosis. (C) and (D) TEM of C. getuliensis particles inside vesicles that strong resemble phagosomes.