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. 2018 Mar 5;8:3994. doi: 10.1038/s41598-018-22327-4

Figure 3.

Figure 3

Ex vivo exposure of WBC to Stx2 confers capacity to cause HUS in recipient mice. Peripheral blood WBC, RBC and PLT, as well as bone marrow (BM) cells, were isolated from LPS-primed mice without HUS. The cells were then incubated ex vivo with Stx2 (10 ng/ml in PBS, experimental group) or saline (PBS, control group) for 30 min (25 °C), followed by thorough wash and transfer (i.v.) into other LPS-primed recipient mice (n = 6 for experimental group, n = 4 for control group). (A) The experimental scheme. (B–D) HUS development in recipient mice assessed by body weight loss (B), thrombocytopenia (C) and renal dysfunction (D). Data represent four individual experiments; *P < 0.05, **P < 0.01, ***P < 0.001 versus respective controls.