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. 2018 Mar 5;8:4011. doi: 10.1038/s41598-018-22295-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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3D histological data from optically-clear tissue at macro- and microscale. CLARITY-processing combined with immunohistochemistry and confocal microscopy allowed whole brain imaging at cellular resolution. (A) GFAP-positive astrocytes (green), parvalbumin-positive interneurons (red) and tomato lectin-positive blood vessels (white) with DAPI stained cell nuclei (blue) in a cerebral hemisphere from an adult mouse brain. (B) High magnification image of boxed region from (A) shows the resolution of the image. (C and D) Individual channels were separated to show regional differences in cellular distribution, (C) green channel - GFAP positive astrocytes are particularly dense within the hippocampus and white matter, (D) red channel - parvalbumin positive interneurons are predominantly found within the cortex and pyramidal layers of the hippocampaus. Scale bar: A,C and D = 1 mm, B = 100 μm.