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. 2018 Mar 5;8:3990. doi: 10.1038/s41598-018-22400-y

Figure 3.

Figure 3

ApoE3 increases CLCF1-induced CNTFR activation. (A) Ba/F3 transfected with CNTFRα, gp130 and LIFRβ cDNA were successively incubated with mouse biotinylated CLCF1 (1 μg/ml) alone or in the presence of ApoE3 (10 mg/ml) and PE-labelled streptavidin and fluorescence was assessed by flow cytometry. Vehicle: cells incubated with PE-streptavidin alone. (B) Ba/F3-CNTFR were stimulated with CLCF1 (100 ng/ml) or ApoE3 (10 mg/ml) alone or in combination for 15 min. STAT3 tyrosine phosphorylation was assessed by intracellular staining with anti-pSTAT3 mAb and flow cytometry. Vehicle: cells incubated with PE-streptavidin alone. Histograms indicate the mean fluorescence intensity of 3 independent samples ± SD.