Fig. 4.

Ligand binding kinetics. a Secondary structure of dGsw^C74U highlighting the C to U mutation at position 74 in red to accommodate the fluorescence ligand analog 2-aminopurine-2′deoxyriboside (2′dAP). b Final k_on and c k_off rates of 2′dAP binding to dGsw^C74U derived from data shown in Supplementary Fig. 4 and Table 3 at varying transcript lengths. Errors correspond to the s.d. of the fit