Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Mar 5;8:4025. doi: 10.1038/s41598-018-22391-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

HLA-II-moDCs polarize CD4⁺ naive T cells into a Tfh phenotype. In all experiments, CD4⁺ naive T cells were cocultured with stimulated moDCs for 6 days. (A) Flow cytometry analysis of CXCR5 surface expression (gated on CD4⁺cells). One representative experiment out of 9 performed is shown. (B) Summary analysis of the CXCR5 percentage expression. Each line represents a single experiment (n = 9). (C) Cell culture supernatants were analyzed for IL-21 production by ELISA (mean ± SEM, n = 9). (D) Summarized data for intracellular IL-21 levels by flow cytometry. Values are expressed as the fold increase in IL-21 levels compared with levels in untreated cells (mean ± SEM, n = 13). (E) Flow cytometry analysis for Th1 (CXCR3), Th2 (CCR4) and Th17 (CCR6) surface marker expression (gated on CD4⁺ cells). One representative experiment of 9 was shown. (F) Coculture supernatants were analyzed for IFN-γ, IL-4 and IL-17A levels by ELISA. Values are expressed as fold increase in cytokine levels compared with levels in unstimulated cells (mean ± SEM, n = 9). (G) Flow cytometry analysis for the surface expression of ICOS and the intracellular expression of Bcl-6 and pSTAT3 in CD4⁺45RA⁺ T cells stimulated with moDCs for 6 days (mean ± SEM, n ≥ 4). *p < 0.05; NS, non-stimulated; IgG, isotype control.