Table 4.
PCR primers used in this study.
| Genes | Primers | Sequences (5′–3′) | Amplification length |
|---|---|---|---|
| tcdA | tcdA-F | GGACATGGTAAAGATGAATTC | 546 bp |
| tcdA-R | CCCAATAGAAGATTCAATATTAAGCTT | ||
| tcdB | tcdB-F | GTGTAGCAATGAAAGTCCAAGTTTACGC | 204 bp |
| tcdB-R | CACTTAGCTCTTTGATTGCTGCACCT | ||
| tcdC | tcdC-F | TCTCTACAGCTATCCCTGGT | 673 bp |
| tcdC-R | AAAAATGAGGGTAACGAATTT | ||
| tcdR | tcdR-F | CTCAGTAGATGATTTGCAAGAA | 473 bp |
| tcdR-R | TTTTAAATGCTCTATTTTTAGCC | ||
| tcdE | tcdE-F | AGGAGGCGTTATGAATATGA | 358 bp |
| tcdE-R | TGGTAATCCACATAAGCACA | ||
| cdtA | cdtA-F | TGAACCTGGAAAAGGTGATG | 375 bp |
| cdtA-R | AGGATTATTTACTGGACCATTTG | ||
| cdtB | cdtB-F | CTTAATGCAAGTAAATACTGAG | 510 bp |
| cdtB-R | AACGGATCTCTTGCTTCAGTC | ||
| 16SrRNA | 27 F | AGAGTTTGATCCTGGCTCAG | 1465 bp |
| 1492 R | TACGGCTACCTTGTTACGACTT | ||
| 16–23 S rDNA | PRB | GTGCGGCTGGATCACCTCCT | variable |
| PRBas | CCCTGCACCCTTAATAACTTGACC | ||
| adk | adk-P1 | GCTATGGTGCCAGCTCTTAC | 1500 bp |
| adk-P2 | GACCAGGTGAGCCTACTATG |