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. 2018 Mar 5;9:938. doi: 10.1038/s41467-018-03118-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Centrosome movements upon depletion of cortical and nuclear dynein reveal centering force dynamics. a, b Snapshots and schematics of centrosome centration in zyg-12(ct350) goa-1/gpa-16(RNAi) embryos. Since pronuclear meeting does not occur in zyg-12(ct350) goa-1/gpa-16(RNAi) embryos, in this figure time 0 s is defined as the half-centration time (indicated by the green lettering on the x axis in c and d; Methods). In b of this and subsequent figures, the red crosses represent depleted dynein motors. c, d Pronuclei and centrosome midpoint positions along the A–P axis as a function of time in eight zyg-12(ct350) goa-1/gpa-16(RNAi) embryos (c), as well as their average, represented with S.E.M. d Black-dashed line in d: fit with sigmoidal model (Eq. 4—Methods, χ² = 27, P = 0.99)