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. 2018 Mar 5;8:4012. doi: 10.1038/s41598-018-22159-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison between ZIKV 1107 and ZIKV NS5. (A) Urine and (B) blood samples were subjected to ZIKV qRT-PCR detection. Bar-charts show the number of samples which are PCR positive or negative. Using mean C_t value = 40 as cut-off, extrapolated ZIKV RNA copies are: ZIKV 1107–0.601, ZIKV NS5–1.497. (C) Overall agreement between ZIKV 1107 and ZIKV NS5 was assessed by κappa test for (C) urine and (D) blood samples. Statistical significance was measured using 2-sided Fisher exact test between the number of samples tested ZIKV positive by ZIKV 1107 or ZIKV NS5. PPV, positive predictive value; NPV, negative predictive value shown with 95% confidence interval.