Figure 3.

Effect of VPA on (A) iNOS and (B) IL-1β mRNA expression in iron-rich cultures of activated microglia. Microglial cells were plated in 24-well plates. After 6 h of the indicated treatments, mRNA was isolated and iNOS and IL-1β gene expression were assayed by semi-quantitative reverse transcription polymerase chain reaction. **P<0.01 vs. control; ^##P<0.01 vs. LPS + iron. VPA, valproic acid; iNOS, inducible nitric oxide synthase; IL-1β, interleukin 1β; LPS, lipopolysaccharide; FCA, ferric citrate ammonium; DAU, densitometry arbitrary unit.