Table 4.
Comparative effects of lipid-coated ZnO (Shield Zn [SZ]) vs uncoated ZnO on gene expressions in the jejunal mucosa of weanling pigs
| Variable | ZnO1) | SZ1) | SEM | p-value | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
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|
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| 100 (NC) | 2,500 (PC) | 100 | 200 | 400 | Contrast | Regression3) | ||||
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| NC2) | PC2) | L | Q | |||||||
| IGF-I | 1.00 | 2.09 | 0.58 | 1.27 | 2.21 | 0.54 | 0.572 | 0.248 | 0.003 | 0.004 |
| ZO-1 | 1.00 | 3.59 | 1.16 | 2.03 | 3.01 | 1.39 | 0.511 | 0.351 | 0.142 | 0.157 |
| Occludin | 1.00 | 1.99 | 0.95 | 0.53 | 0.72 | 0.39 | 0.562 | 0.009 | 0.605 | 0.743 |
| TNF-α | 1.00 | 1.70 | 0.39 | 1.17 | 1.33 | 0.56 | 0.951 | 0.261 | 0.163 | 0.210 |
| IL-6 | 1.00 | 1.28 | 2.31 | 0.99 | 0.65 | 0.38 | 0.477 | 0.929 | 0.017 | 0.033 |
| IL-10 | 1.00 | 2.87 | 0.37 | 1.12 | 1.92 | 0.99 | 0.906 | 0.141 | 0.053 | 0.062 |
| TGF-β1 | 1.00 | 2.52 | 0.87 | 1.07 | 1.47 | 0.36 | 0.740 | 0.002 | 0.119 | 0.123 |
NC, negative control; PC, positive control; SEM, standard error of mean; L, linear; Q, quadratic; IGF-I, insulin-like growth factor-I; ZO-1, zonula occludens protein-1; TNF-α, tumor necrosis factor-α; IL-6, interleukin-6; TGF-β1, transforming growth factor-β1.
1)
The numeral under the column heading indicates the dietary Zn concentration in mg/kg provided by the Zn supplement. Data are the means of 8 piglets.
2)
Total SZ (SZ-100, -200, and -400) vs. NC and total SZ vs PC, respectively.
3)
Linear and quadratic regressions on the dietary SZ concentration within the total SZ group piglets were analyzed separately.