Figure 6.

FP‐binding nanobodies like the GFP‐binding GBP enable the combinatorial analysis of proteins and their interaction partners through imaging and mass spectrometry‐based proteomics. A protein of interest (Protein 1) expressed as an FP fusion can be imaged using conventional methods. A nanobody that binds the FP is chemically immobilized on a solid support, which facilitates enrichment of the target protein and any interacting protein (Proteins 2 and 3). Subsequent MS analysis enables the identification and assignment of the co‐enriched interacting proteins.