Figure 3.

Anti‐ERp72 diminishes platelet granule secretion. P‐selectin exposure following alpha‐granule secretion in human platelets (A, n = 3) was measured by flow cytometry. Platelets (2 × 10⁸ cells mL ⁻¹) were incubated with control antibody (25 μg mL⁻¹, open histogram) or anti‐ERp72 (25 μg mL⁻¹, closed histogram) for 5 minutes prior to stimulation with CRP‐XL (0.5 μg mL⁻¹). (B) Mouse platelet alpha‐granule secretion in the presence of 25 or 50 μg mL⁻¹ anti‐ERp72 or control antibody was stimulated using thrombin (0.1U mL⁻¹); n = 4. For dense granule secretion assays, platelets (4 × 10⁸ cells mL⁻¹) were pre‐incubated with either control antibody (25 μg mL⁻¹) or anti‐ERp72 (10–25 μg mL⁻¹) for 5 min prior to stimulation with collagen (1 μg mL⁻¹) and ATP secretion recorded for 180 s (C); n = 3. Graphs represent mean ± SEM. P values were calculated by Student's t‐test (A) or one‐way anova (B and C) (*P < 0.05, **P < 0.01).