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. 2018 Jan 5;93(3):909–917. doi: 10.1002/jctb.5519

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© 2017 The Authors. Journal of Chemical Technology & Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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JCTB-5519-FIG-0002-c — Fluorescence spectrum of dyes with mAb aggregates spiked into buffer. Thermally stressed IgG1 mAb A spiked into non‐aggregated mAb. Concentration of antibody in each well was 1 mg mL^‐1. This was performed in triplicate. (A) 50 µmol L^‐1 Bis‐ANS, ex/em‐ 390/450–600 nm, gain 70; (B) 5X SYPRO Orange, ex/em‐ 495/550–700 nm, gain 100; (C) 3 µM ProteoStat, ex/em‐ 530/560–700 nm; (D) 50 µmol L^‐1 Thioflavin T, ex/em‐ 460–600 nm, gain 110. 0% spiked aggregates 10 % spiked aggregates Buffer Blank.

Inline graphic — Fluorescence spectrum of dyes with mAb aggregates spiked into buffer. Thermally stressed IgG1 mAb A spiked into non‐aggregated mAb. Concentration of antibody in each well was 1 mg mL^‐1. This was performed in triplicate. (A) 50 µmol L^‐1 Bis‐ANS, ex/em‐ 390/450–600 nm, gain 70; (B) 5X SYPRO Orange, ex/em‐ 495/550–700 nm, gain 100; (C) 3 µM ProteoStat, ex/em‐ 530/560–700 nm; (D) 50 µmol L^‐1 Thioflavin T, ex/em‐ 460–600 nm, gain 110. 0% spiked aggregates 10 % spiked aggregates Buffer Blank.