Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 5;93(3):909–917. doi: 10.1002/jctb.5519

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 The Authors. Journal of Chemical Technology & Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

JCTB-5519-FIG-0008-c — SEC of mAb and null clarified cell cultures with SYPRO Orange and ProteoStat. (A) Null cell line shake flask 1 with 5× SYPRO Orange; (B) IgG mAb B with 5× SYPRO Orange; (C) null cell line shake flask 1 with 3 µmol L^‐1 ProteoStat; (D) IgG mAb B with 3 µmol L^‐1 ProteoStat. Clarified cell culture samples were combined with dye before loading onto the column. A TSKgel2000SWI column (7.8×300 mm) with a flow rate of 0.5 mL min^‐1 with a fluorescence detector was used. SYPRO Orange samples ex/em 495/590 nm, ProteoStat samples ex/em 530/605 nm. Running buffer composed of 100 mmol L^‐1 sodium phosphate (monobasic), 400 mmol L^‐1 sodium chloride, pH 6.8. Injection volume of 50 µL. Media Day 10 Day 14.

Inline graphic — SEC of mAb and null clarified cell cultures with SYPRO Orange and ProteoStat. (A) Null cell line shake flask 1 with 5× SYPRO Orange; (B) IgG mAb B with 5× SYPRO Orange; (C) null cell line shake flask 1 with 3 µmol L^‐1 ProteoStat; (D) IgG mAb B with 3 µmol L^‐1 ProteoStat. Clarified cell culture samples were combined with dye before loading onto the column. A TSKgel2000SWI column (7.8×300 mm) with a flow rate of 0.5 mL min^‐1 with a fluorescence detector was used. SYPRO Orange samples ex/em 495/590 nm, ProteoStat samples ex/em 530/605 nm. Running buffer composed of 100 mmol L^‐1 sodium phosphate (monobasic), 400 mmol L^‐1 sodium chloride, pH 6.8. Injection volume of 50 µL. Media Day 10 Day 14.