Figure 2.

(A,B) SplitsTree plots of BoNT and NTNH protein sequences respectively. TeNT protein is not associated with an NTNH accessory protein. Both plots show that BoNT/X and eBoNT/J and their associated NTNH are more closely related to each other than they are to all other proteins. (C,D). SimPlot analyses of BoNT and NTNH protein domain sequences, respectively, using eBoNT/J and its NTNH as seeds; main functional domains of each eBoNT/J cluster protein are outlined above. The closer relatedness of BoNT/X to eBoNT/J continues throughout its length; however, the NTNH associated with eBoNT/J is almost equally distant from all other examples.