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. 2018 Jan 18;244(3):311–322. doi: 10.1002/path.5013

Figure 2.

path-5013-fig-0002-c

MMP‐3 is expressed in human respiratory epithelial cells in TB patients and MMP‐3 gene expression and secretion are driven by IL‐17. (A) Pulmonary epithelial cells adjacent to TB granulomas express MMP‐3. The figure is representative of lung biopsies from five patients with TB (scale bar = 20 μm). (B) Minimal staining for MMP‐3 was observed in five normal lung specimens (scale bar = 20 μm). (C) Kinetic experiments in NHBE cells demonstrated that MMP‐3 secretion increased progressively until 72 h after stimulation with 10 ng/ml IL‐17 (p < 0.001). (D) IL‐17‐driven MMP‐3 mRNA accumulation peaked at 24 h, rising to four‐fold above baseline (p < 0.01). (E) Direct infection of NHBE cells with Mtb at an MOI from 0.1 to 10 did not alter MMP‐3 secretion and there was no synergy between Mtb infection and IL‐17 stimulation.