Skip to main content
. 2018 Jan 18;244(3):311–322. doi: 10.1002/path.5013

Figure 5.

path-5013-fig-0005-b

IL‐17 regulation of MMP‐3 secretion is p38 MAP kinase‐dependent. (A) Phosphorylation of p38 after 30 min of stimulation was increased by both CoMTb and IL‐17. p38 phosphorylation was significantly increased when cells were co‐stimulated with IL‐17 and CoMTb. There was no change in total p38. Densitometric analysis was performed using Scion image software. (B) MMP‐3 secretion and (C) MMP‐3 mRNA accumulation driven by IL‐17 was inhibited in a concentration‐dependent manner by SB203580, a specific p38 chemical inhibitor (p < 0.001 for both protein and mRNA). (D) Transfection of the epithelial cells with p38 siRNA decreased IL‐17/CoMTb‐driven MMP‐3 secretion by 50% (p < 0.01). The suppression was maximal with 30 nm of siRNA (E) Similarly, IL‐17‐driven MMP‐3 mRNA decreased to baseline by siRNA inhibition of p38 (p < 0.01). Cells were also transfected with non‐targeting (NT) siRNA, which had no effect.