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. 2018 Jan 18;244(3):311–322. doi: 10.1002/path.5013

Figure 6.

path-5013-fig-0006-b

Epithelial MMP‐3 secretion is regulated by the PI3K p110α subunit and AKT. (A) IL‐17‐dependent MMP‐3 secretion was reduced to baseline after incubation of NHBE cells with LY294002, a non‐specific PI3K proximal subunit inhibitor (p < 0.0001). Suppression was maximal at 10 μm. (B) siRNA transfection of NHBE cells identified that the p110α subunit was key in this suppression (p < 0.0001). MMP‐3 mRNA was suppressed to baseline with 30 nm siRNA. Cells were also transfected with non‐targeting (NT) siRNA, which had no effect. (C) Downstream, inhibition of AKT by a specific inhibitor (AKT inhibitor VIII) resulted in MMP‐3 suppression to baseline. The effect was dose‐dependent and maximal at 2.5 μm of the AKT inhibitor (p < 0.0001).