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. 2018 Mar 5;37:43. doi: 10.1186/s13046-018-0714-6

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — The level of extracellular calcium influx is positively correlated with TNFα-mediated apoptosis. a Apoptosis analysis by flow cytometry 24 h after treatment as indicated. b Confocal microscope analysis of [Ca²⁺]_c level using fluorescent probe Fura-2/AM in 10 kinds of HCC cells with treatment as indicated. c Correlational analysis of the maximal increased level of cytosolic Ca²⁺ and apoptotic rates in HCC cells after TNFα (100 ng/mL) treatment. Data were shown as mean ± SD. All experiments were performed at least three times